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| Kawase, Osamu; Nishikawa, Yoshifumi; Bannai, Hiroshi; Matsuo, Tomohide; Xuan, Xuenan. |
| Toxoplasma gondii is an obligate intracellular protozoan parasite that invades a wide range of host cells. Upon encountering host cells, the parasite releases a large variety of proteins from secretory organelles, such as micronemes, rhoptries and dense granules. The secretion of microneme protein is essential for parasite invasion. We found that a secreted protein with an altered thrombospondin repeat of Toxoplasma gondii (TgSPATR) was a novel microneme protein, which was different from known microneme proteins that carry thrombospondin repeat domains. TgSPATR was secreted in response to an intra-parasitic elevation of Ca2+ and probably secreted during early stages of parasite invasion. Thus, we suggested that TgSPATR, new member of microneme secretory... |
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Palavras-chave: Ca2+-dependent secretion; Microneme protein; Secreted protein with an alteredthrombospondin repeat; Toxoplasma gondii. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2819 |
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| Zhang, Houshuang; Lee, Eung-goo; Liao, Min; Compaore, Muller K.A.; Zhang, Guohong; Kawase, Osamu; Fujisaki, Kozo; Sugimoto, Chihiro; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| The characterization of the cross-reactive antigens of two closely related apicomplexan parasites, Neospora caninum and Toxoplasma gondii, is important to elucidate the common mechanisms of parasite–host interactions. In this context, a gene encoding N. caninum ribosomal phosphoprotein P0 (NcP0) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera from mice immunized with T. gondii tachyzoites. The NcP0 was encoded by a gene with open reading frame of 936 bp, which encoded a protein of 311 amino acids. The NcP0 gene existed as a single copy in the genome and was interrupted by a 432 bp intron. The NcP0 showed 94.5% amino acid identity to T. gondii P0 (TgP0). Anti-recombinant NcP0 (rNcP0) sera recognized a... |
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Palavras-chave: Neospora caninum; Toxoplasma gondii; Ribosomal phosphoprotein P0; Cross-reactive. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1035 |
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